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Pages: 1 Dear allI am confused regarding the calculation of dilution factor. As far as I know, dilution factor = volume of sample/total volume and CFU = (#colony x dilution factor)/volume plated in mL. For example, if I added 1g of sample into 9mL of broth - 10^-1, and transfer 1mL from 10^-1 to second tube (9mL as well). Then this will be 10^-2. Thus, if 1000mL of sample in tube 11 plated onto the agar and I obtained 30 colonies in this plate, CFU = (30 x 10^-11)/1000mL Well, now came to my confusion partWhat if I added 1g of sample directly to 99mL of broth, what is the dilution factor here?
The Chakra Sounds meditation is a very soft way to activate and harmonize the energy centers of the body, working with the vibrations of musical sound.The music was recorded during a live performance of musicians and singers working very closely with the makers of the meditation. In this way, the music not only uses the exact sounds which willactivate each chakra, but also musically illustrates the character of each individual center.The meditator is advised to sing along and harmonize with the music.
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Nation’s first rapid-response emergency homeless shelter provides families specialized and holistic servicesThe Salvation Army, in partnership with The City of Chicago, opened the nation’s first rapid-response emergency homeless assessment and response center today, providing intake services and interim care for families in need. The City's first rapid response center, which was proposed by Mayor Rahm Emanuel, is the latest in a series of innovative initiatives to address homelessness.“As a city, we are committed to providing these essential services to the residents of Chicago who are most in need.” said Mayor Rahm Emanuel.
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